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eclipse-80i upright epifluorescence microscope  (Nikon)


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    Structured Review

    Nikon eclipse-80i upright epifluorescence microscope
    Eclipse 80i Upright Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epifluorescence+microscope+eclipse+80i/pmc10103300__nl3c00377_si_001-19-15-14
    Average 90 stars, based on 1 article reviews
    eclipse-80i upright epifluorescence microscope - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Prostaglandin EP3 receptor-expressing preoptic neurons bidirectionally control body temperature via tonic GABAergic signaling.
    Article Snippet: .. The sections were observed under an epifluorescence microscope (Eclipse 80i, Nikon) or a confocal laser scanning microscope (TCS SP8, Leica). ..

    Article Title: Age-related ciliopathy: Obesogenic shortening of melanocortin-4 receptor-bearing neuronal primary cilia.
    Article Snippet: The sections were further incubated with Alexa594-conjugated streptavidin (5 mg/ml; S11227, Thermo Fisher Scientific) for 1 h. After fluorescence staining, sections were thoroughly washed and mounted onto glass slides. .. Following coverslipped with 50% glycerol/50% PBS containing 2.5% triethylenediamine, they were observed under an epifluorescence microscope (Eclipse 80i, Nikon) or a confocal laser-scanning microscope (TCS SP8, Leica). .. In situ hybridization combined with immunohistochemistry For histochemical measurement of intracellular Cilk1 mRNA (Figure S7C), we performed fluorescence in situ hybridization for Cilk1 mRNA using RNAscopeMultiplex Fluorescent Reagent Kit v2 (323100, Advanced Cell Diagnostics, Newark, CA) combined with immunohistochemistry to detect EGFP and DsRed.

    Article Title: The apoptotic inhibitor z-DEVD-fmk improves the viability and maturation rates of Iberian red deer oocytes while reducing apoptotic markers
    Article Snippet: .. Oocyte nuclei and plasma membrane were observed with an epifluorescence microscope (Eclipse 80i, Nikon Instruments Europe, Amsterdam, The Netherlands) and classified into the following categories: viable (Annexin V−/ EthD-III −), early apoptotic (Annexin V+/ EthD-III−), and dead (Annexin V−/ EthD-III+ and Annexin V+/ EthD-III +). .. Caspase-3 activity was determined in 72 matured oocytes (4 replicates) using NucView® 488 Caspase-3 Assay Kit for live cells (Biotium©, San Francisco, USA; #30029-T).

    Article Title: Transfer printing of perovskite nanocrystal self-assembled monolayers via controlled surface wettability.
    Article Snippet: .. Throughout this study, pure water (γLV1 = 72.8 mN m −1, γdLV1 = 21.8 mN m−1, γhLV1 = 51.0 mN m −1) and Ch2I2 (γLV2 = 50.8 mN m−1, γdLV2 = 50.8 mN m −1, γhLV2 = 0 mN m −1) were used as probe liquids.24,25 Fluorescence microscope observation and PL measurement Fluorescence microscope images and PL spectra of the NC films were collected by using an epifluorescence microscope (ECLIPSE 80i, Nikon, Japan) with a mercury lamp, an excitation filter (400–440 nm), and a PMA-12 photonic multichannel analyzer (Hamamatsu, Japan). ..

    Article Title: Effects of ergothioneine supplementation on meiotic competence and porcine oocyte development
    Article Snippet: The sections were then treated with an anti-bleaching solution (Slow-Fade; Molecular Probes Inc., Eugene, OR, USA), mounted on a glass slide, and sealed with clear nail polish. .. The labeled oocytes were examined using an epifluorescence microscope (Eclipse 80i; Nikon, Tokyo, Japan) equipped with epifluorescence illumination. ..

    Article Title: SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration
    Article Snippet: .. Fluorescence imaging was carried out using an epifluorescence microscope (Eclipse 80i, Nikon) with appropriate excitation/emission filters and captured using Metamorph Software. .. Black-Gold II staining was done using the Black-Gold II myelin staining kit (AG105, Merck Inc, USA) as per the manufacturer’s protocol.

    Article Title: Control of Lateral Assembly and Vertical Stacking in Spin-Coated Lead Halide Perovskite Nanocrystal Films for Enhanced Photoluminescence Efficiency
    Article Snippet: Lead halide perovskite (LHP) nanocrystals (NCs) hold great promise for advanced photonic and optoelectronic applications due to their near-unity photoluminescence (PL) quantum efficiency, narrow emission line width, and tunable spectral wavelength.. However, the fabrication of spatially uniform, ultrathin films over a large-scale device area has been impeded by the instability of LHP NCs toward heat and polar solvents.. Here, we demonstrate a feasible strategy that enables not only the assembly of various LHP NCs for constructing two-dimensional (2D) films but also precise controllability of multilayer films over large-scale areas.

    Article Title: SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration
    Article Snippet: .. The slides were mounted using a mounting medium containing DAPI (Vector Labs) and observed using an epifluorescence microscope (Eclipse 80i, Nikon) using appropriate filters and captured using Metamorph software. ..

    Laser-Scanning Microscopy:

    Article Title: Prostaglandin EP3 receptor-expressing preoptic neurons bidirectionally control body temperature via tonic GABAergic signaling.
    Article Snippet: .. The sections were observed under an epifluorescence microscope (Eclipse 80i, Nikon) or a confocal laser scanning microscope (TCS SP8, Leica). ..

    Article Title: Age-related ciliopathy: Obesogenic shortening of melanocortin-4 receptor-bearing neuronal primary cilia.
    Article Snippet: The sections were further incubated with Alexa594-conjugated streptavidin (5 mg/ml; S11227, Thermo Fisher Scientific) for 1 h. After fluorescence staining, sections were thoroughly washed and mounted onto glass slides. .. Following coverslipped with 50% glycerol/50% PBS containing 2.5% triethylenediamine, they were observed under an epifluorescence microscope (Eclipse 80i, Nikon) or a confocal laser-scanning microscope (TCS SP8, Leica). .. In situ hybridization combined with immunohistochemistry For histochemical measurement of intracellular Cilk1 mRNA (Figure S7C), we performed fluorescence in situ hybridization for Cilk1 mRNA using RNAscopeMultiplex Fluorescent Reagent Kit v2 (323100, Advanced Cell Diagnostics, Newark, CA) combined with immunohistochemistry to detect EGFP and DsRed.

    Clinical Proteomics:

    Article Title: The apoptotic inhibitor z-DEVD-fmk improves the viability and maturation rates of Iberian red deer oocytes while reducing apoptotic markers
    Article Snippet: .. Oocyte nuclei and plasma membrane were observed with an epifluorescence microscope (Eclipse 80i, Nikon Instruments Europe, Amsterdam, The Netherlands) and classified into the following categories: viable (Annexin V−/ EthD-III −), early apoptotic (Annexin V+/ EthD-III−), and dead (Annexin V−/ EthD-III+ and Annexin V+/ EthD-III +). .. Caspase-3 activity was determined in 72 matured oocytes (4 replicates) using NucView® 488 Caspase-3 Assay Kit for live cells (Biotium©, San Francisco, USA; #30029-T).

    Membrane:

    Article Title: The apoptotic inhibitor z-DEVD-fmk improves the viability and maturation rates of Iberian red deer oocytes while reducing apoptotic markers
    Article Snippet: .. Oocyte nuclei and plasma membrane were observed with an epifluorescence microscope (Eclipse 80i, Nikon Instruments Europe, Amsterdam, The Netherlands) and classified into the following categories: viable (Annexin V−/ EthD-III −), early apoptotic (Annexin V+/ EthD-III−), and dead (Annexin V−/ EthD-III+ and Annexin V+/ EthD-III +). .. Caspase-3 activity was determined in 72 matured oocytes (4 replicates) using NucView® 488 Caspase-3 Assay Kit for live cells (Biotium©, San Francisco, USA; #30029-T).

    Ethidium Homodimer Assay:

    Article Title: The apoptotic inhibitor z-DEVD-fmk improves the viability and maturation rates of Iberian red deer oocytes while reducing apoptotic markers
    Article Snippet: .. Oocyte nuclei and plasma membrane were observed with an epifluorescence microscope (Eclipse 80i, Nikon Instruments Europe, Amsterdam, The Netherlands) and classified into the following categories: viable (Annexin V−/ EthD-III −), early apoptotic (Annexin V+/ EthD-III−), and dead (Annexin V−/ EthD-III+ and Annexin V+/ EthD-III +). .. Caspase-3 activity was determined in 72 matured oocytes (4 replicates) using NucView® 488 Caspase-3 Assay Kit for live cells (Biotium©, San Francisco, USA; #30029-T).

    Fluorescence:

    Article Title: Transfer printing of perovskite nanocrystal self-assembled monolayers via controlled surface wettability.
    Article Snippet: .. Throughout this study, pure water (γLV1 = 72.8 mN m −1, γdLV1 = 21.8 mN m−1, γhLV1 = 51.0 mN m −1) and Ch2I2 (γLV2 = 50.8 mN m−1, γdLV2 = 50.8 mN m −1, γhLV2 = 0 mN m −1) were used as probe liquids.24,25 Fluorescence microscope observation and PL measurement Fluorescence microscope images and PL spectra of the NC films were collected by using an epifluorescence microscope (ECLIPSE 80i, Nikon, Japan) with a mercury lamp, an excitation filter (400–440 nm), and a PMA-12 photonic multichannel analyzer (Hamamatsu, Japan). ..

    Article Title: SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration
    Article Snippet: .. Fluorescence imaging was carried out using an epifluorescence microscope (Eclipse 80i, Nikon) with appropriate excitation/emission filters and captured using Metamorph Software. .. Black-Gold II staining was done using the Black-Gold II myelin staining kit (AG105, Merck Inc, USA) as per the manufacturer’s protocol.

    Labeling:

    Article Title: Effects of ergothioneine supplementation on meiotic competence and porcine oocyte development
    Article Snippet: The sections were then treated with an anti-bleaching solution (Slow-Fade; Molecular Probes Inc., Eugene, OR, USA), mounted on a glass slide, and sealed with clear nail polish. .. The labeled oocytes were examined using an epifluorescence microscope (Eclipse 80i; Nikon, Tokyo, Japan) equipped with epifluorescence illumination. ..

    Imaging:

    Article Title: SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration
    Article Snippet: .. Fluorescence imaging was carried out using an epifluorescence microscope (Eclipse 80i, Nikon) with appropriate excitation/emission filters and captured using Metamorph Software. .. Black-Gold II staining was done using the Black-Gold II myelin staining kit (AG105, Merck Inc, USA) as per the manufacturer’s protocol.



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